Controllo della attività di ATM da parte di RNA generati da DICER e DROSHA
- 3 Anni 2012/2015
- 210.600€ Totale Fondi
When studying the mechanisms of action of ATM, the protein mutated in AT patients, we unexpectedly discovered that RNA is essential for its proper functioning. More specifically we found that at DNA double-strand breaks (the events that trigger ATM activation) long molecules of RNA are generated that are then processed in shorter ones. These RNAs then pair between them and this pairing is essential for the activation of ATM. This discovery reveals for the first time an unexpected level of regulation of this protein and a new mechanism of protection of the integrity of the genome.
Pubblicazioni Scientifiche
- 2017-04-01 AGING CELL
A novel single-cell method provides direct evidence of persistent DNA damage in senescent cells and aged mammalian tissues
- 2015-07-03 CELL CYCLE
Human nuclear ARGONAUTE 2 interacts in vivo only with small RNAs and not with DNA
- 2017-07-01 CELL DEATH & DISEASE
The cohesin complex prevents Myc-induced replication stress
- 2012-11-01 CELL DEATH AND DIFFERENTIATION
Differential regulation of DNA damage response activation between somatic and germline cells in Caenorhabditis elegans
- 2014-06-01 CELL DEATH AND DIFFERENTIATION
Oncogene-induced reactive oxygen species fuel hyperproliferation and DNA damage response activation
- 2021-01-05 CELL REPORTS
MRE11-RAD50-NBS1 Complex Is Sufficient to Promote Transcription by RNA Polymerase II at Double-Strand Breaks by Melting DNA Ends
- 2019-01-01 CELLULAR SENESCENCE
DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA)
- 2019-01-01 CELLULAR SENESCENCE
DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA) (vol 1896, pg 11, 2020)
- 2018-04-25 CHEMICAL REVIEWS
From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond
- 2014-06-01 CURRENT OPINION IN GENETICS & DEVELOPMENT
Irreparable telomeric DNA damage and persistent DDR signalling as a shared causative mechanism of cellular senescence and ageing
- 2015-07-31 ELIFE
Resection is responsible for loss of transcription around a double-strand break in Saccharomyces cerevisiae
- 2015-05-12 EMBO JOURNAL
Telomerase abrogates aneuploidy-induced telomere replication stress, senescence and cell depletion
- 2015-02-01 EMBO REPORTS
RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA
- 2017-07-01 FEBS JOURNAL
Express or repress? The transcriptional dilemma of damaged chromatin
- 2016-04-01 JOURNAL OF CELL SCIENCE
DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors
- 2017-10-27 JOURNAL OF MOLECULAR BIOLOGY
Transcription and DNA Damage: Holding Hands or Crossing Swords?
- 2012-04-01 NATURE CELL BIOLOGY
Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation
- 2017-12-01 NATURE CELL BIOLOGY
Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks
- 2017-02-27 NATURE COMMUNICATIONS
DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs
- 2017-05-31 NATURE COMMUNICATIONS
A damaged genome's transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks
- 2018-12-18 NATURE COMMUNICATIONS
BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment
- 2018-04-01 NATURE PROTOCOLS
Target-enrichment sequencing for detailed characterization of small RNAs
- 2019-05-01 NATURE PROTOCOLS
RNase A treatment and reconstitution with DNA damage response RNA in living cells as a tool to study the role of non-coding RNA in the formation of DNA damage response foci
- 2015-05-01 NATURE STRUCTURAL & MOLECULAR BIOLOGY
Notch is a direct negative regulator of the DNA-damage response
- 2014-10-23 PLOS ONE
Stable Cellular Senescence Is Associated with Persistent DDR Activation
- 2014-03-01 TRENDS IN CELL BIOLOGY
A direct role for small non-coding RNAs in DNA damage response
